human normal hepatocytes wrl 68  (ATCC)

Journal: Open Life Sciences

Article Title: SPOP regulates the expression profiles and alternative splicing events in human hepatocytes

doi: 10.1515/biol-2022-0755

Figure Lengend Snippet: Overexpression of SPOP could facilitate the proliferation of human normal hepatocytes (WRL68). (a) Transfection efficiency of SPOP in WRL68 cells was measured by qRT-PCR. (b) Proliferative abilities of WRL68 cells in the SPOP group and the vec group were detected by CCK8 assay. (c) Colony formation assay detected the proliferations of WRL68 cells in the SPOP group and the vec group. * p < 0.05, ** p < 0.01.

Article Snippet: Human normal hepatocytes WRL68 (ATCC, Manassas, USA) were cultured in Dulbecco’s Modified Eagle Medium (cat. no. PM150210, Procell, Wuhan, China) with 10% GibcoTM fetal bovine serum (cat. no. 10099141C, Invitrogen, AUS) and 1% penicillin–streptomycin (10,000 U/mL) (cat. no. 15140122, Invitrogen, USA), at 37°C in a 5% CO 2 humidified atmosphere.

Techniques: Over Expression, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay

Journal: Open Life Sciences

Article Title: SPOP regulates the expression profiles and alternative splicing events in human hepatocytes

doi: 10.1515/biol-2022-0755

Figure Lengend Snippet: Overexpression of SPOP could change the gene expression profiles of WRL68. (a) Boxplot of sample gene expression distribution. (b) Correlation between SPOP overexpression (SPOP rep1/2/3) and control (vec rep1/2/3) samples. (c) DEGs between the SPOP group and the vec group. (d) Correlation of log2 fold-change between RNA-Seq and RT-qPCR for significantly DEGs.

Article Snippet: Human normal hepatocytes WRL68 (ATCC, Manassas, USA) were cultured in Dulbecco’s Modified Eagle Medium (cat. no. PM150210, Procell, Wuhan, China) with 10% GibcoTM fetal bovine serum (cat. no. 10099141C, Invitrogen, AUS) and 1% penicillin–streptomycin (10,000 U/mL) (cat. no. 15140122, Invitrogen, USA), at 37°C in a 5% CO 2 humidified atmosphere.

Techniques: Over Expression, Gene Expression, Control, RNA Sequencing, Quantitative RT-PCR

Journal: Open Life Sciences

Article Title: SPOP regulates the expression profiles and alternative splicing events in human hepatocytes

doi: 10.1515/biol-2022-0755

Figure Lengend Snippet: Potential biological functions of SPOP-related genes in WRL68 cells. (a) Top 30 most enriched GO terms of the upregulated DEGs. (b) Top 30 most enriched GO terms of the downregulated DEGs. (c) Top 20 most enriched KEGG pathways of all DEGs. (d) Top 20 most enriched DisGeNET lists of all DEGs.

Article Snippet: Human normal hepatocytes WRL68 (ATCC, Manassas, USA) were cultured in Dulbecco’s Modified Eagle Medium (cat. no. PM150210, Procell, Wuhan, China) with 10% GibcoTM fetal bovine serum (cat. no. 10099141C, Invitrogen, AUS) and 1% penicillin–streptomycin (10,000 U/mL) (cat. no. 15140122, Invitrogen, USA), at 37°C in a 5% CO 2 humidified atmosphere.

Techniques:

Journal: Open Life Sciences

Article Title: SPOP regulates the expression profiles and alternative splicing events in human hepatocytes

doi: 10.1515/biol-2022-0755

Figure Lengend Snippet: Enrichment biological function of SPOP RASEs in WRL68 cells. (a) Number of significant RASEs. (b) GO functional enrichment of different RASEs. (c) KEGG pathway enrichment analysis results: A3SS events, 11 A5SS events, 20 MXE events, 11 RI events, and 100 SE events.

Article Snippet: Human normal hepatocytes WRL68 (ATCC, Manassas, USA) were cultured in Dulbecco’s Modified Eagle Medium (cat. no. PM150210, Procell, Wuhan, China) with 10% GibcoTM fetal bovine serum (cat. no. 10099141C, Invitrogen, AUS) and 1% penicillin–streptomycin (10,000 U/mL) (cat. no. 15140122, Invitrogen, USA), at 37°C in a 5% CO 2 humidified atmosphere.

Techniques: Functional Assay

Journal: Open Life Sciences

Article Title: SPOP regulates the expression profiles and alternative splicing events in human hepatocytes

doi: 10.1515/biol-2022-0755

Figure Lengend Snippet: HCC pathway modified by SPOP overexpression in WRL68 (the figure was drawn according to the information from KEGG PATHWAY database. The molecules in red were upregulated with SPOP overexpression and the molecules in green were downregulated with SPOP overexpression).

Article Snippet: Human normal hepatocytes WRL68 (ATCC, Manassas, USA) were cultured in Dulbecco’s Modified Eagle Medium (cat. no. PM150210, Procell, Wuhan, China) with 10% GibcoTM fetal bovine serum (cat. no. 10099141C, Invitrogen, AUS) and 1% penicillin–streptomycin (10,000 U/mL) (cat. no. 15140122, Invitrogen, USA), at 37°C in a 5% CO 2 humidified atmosphere.

Techniques: Modification, Over Expression

Journal: Cancer gene therapy

Article Title: Gene expression in intrahepatic tumors through DNA recombination by a replication-activated adenovirus vector.

doi: 10.1038/sj.cgt.7700719

Figure Lengend Snippet: Figure 1 Ad.IR-BG-mediated tumor-selective gene expression. Human colon carcinoma cells (LoVo and HT29), hepatocellular carcinoma cells (SMMC7721 and MHCC97), rat hepatocytes (WB), and human colon epithelial cells (NCM) were infected by a mixture of Ad.BG or Ad.IR-BG and Ad.Luc vector (1:1 at MOI 100 each) for 72 hours. Activities for b-gal and luciferase were measured from cell lysates by assay kits as described in Materials and methods. The b- gal expression is expressed as units of activity normalized by luciferase activity for each cell line (U 102). n ¼ 4, *P o 0.05.

Article Snippet: Normal human hepatocytes (WRL68, ATCC) were maintained in DMEM as recommended.

Techniques: Gene Expression, Infection, Plasmid Preparation, Luciferase, Expressing, Activity Assay